Models of calcium dynamics
James Sneyd (2007), Scholarpedia, 2(3):1576. | doi:10.4249/scholarpedia.1576 | revision #137198 [link to/cite this article] |
In very many cell types, the concentration of free intracellular calcium oscillates, with a period ranging from a few seconds to a few minutes. These calcium oscillations are thought to control a wide variety of cellular processes, and are often organised into intracellular and intercellular calcium waves (Tsien and Tsien, 1990; Berridge, 1990, 1997; Berridge et al., 2003; Clapham, 1995; Sneyd et al., 1995; Thomas et al., 1996; Falcke, 2004; Schuster et al., 2002).
When constructing mathematical models of calcium oscillations and waves it is crucial to keep two points firmly in mind:
- Each cell type is different. The mechanisms that underlie calcium oscillations in one cell type might be, and usually are, quite different in detail from the mechanisms underlying oscillations in another cell type. Despite this, there is still a lot in common between cell types, and certain generalisations can be made. Carefully.
- It is a relatively simple matter to write down a system of equations that exhibits oscillations. It is even relatively simple to fiddle the model until the oscillations look like the data. It is, however, much more difficult to use the model to say something interesting and new about the cellular physiology.
Contents |
Calcium Oscillations
Physiological Mechanisms
The resting state
At rest, the concentration of calcium in the cell cytoplasm is low, around 30 nM, while outside the cell, and in the internal compartments such as the endoplasmic reticulum (ER), sarcoplasmic reticulum (SR) and mitochondria it is orders of magnitude higher. These concentration gradients are maintained in part by calcium ATPase pumps, which use the energy of ATP to pump calcium against its gradient, either out of the cell or into internal compartments. Sodium-Calcium exchangers also play an important role in some cell types. These large concentration gradients allow for a very rapid increase in cytosolic calcium concentration when calcium channels are opened.
Different sources of calcium
The increased calcium concentration in an oscillation comes from one of two places:
- From internal stores such as the ER, the SR, the mitochondria, or calcium buffers.
- From outside the cell. Calcium channels in the plasma membrane can be controlled by a variety of things, including voltage, agonist stimulation and the amount of calcium in the ER.
As a general rule, voltage-gated calcium entry occurs in electrically excitable cells, while in non-excitable cells (such as epithelial cells, hepatocytes, or oocytes) calcium release is from internal stores.
Agonist stimulation
Typically in non-excitable cells, binding of an agonist such as a hormone or a neurotransmitter to cell-surface receptors initiates a series of reaction, linked through a G-protein, that ends in the production of the second messenger inositol trisphosphate (IP_{3}), which diffuses through the cytoplasm of cell and binds to IP_{3} receptors (IPR) which are located on the membrane of the ER. IPR are also calcium channels, and when they bind IP_{3} they open, allowing for the fast release of calcium from the ER into the cytoplasm.
Feedback mechanisms
Without some additional feedback mechanisms, simple release of calcium from the ER will not result in oscillations. There are a number of feedback pathways that operate in different cell types to mediate calcium oscillations. The two principal mechanisms are:
- Modulation by calcium of the IPR open probability. In most cell types, release of calcium through the IPR can stimulate the release of additional calcium from the ER, often by binding to the IPR and increasing its open probability. This leads to the autocatalytic release of calcium from the ER, in a process usually called calcium-induced calcium release, or CICR. Calcium can also bind to IPR at a different binding site to inhibit calcium release, leading to a slow negative feedback and termination of calcium release. Calcium can then be taken up into the ER by the ATPase pumps, leading to a decrease in cytoplasmic calcium concentration and resetting of the cycle.
- Modulation by calcium of IP_{3} production and degradation. Since the rates of IP_{3} production and degradation are calcium-dependent this can lead to either positive or negative feedback loops, with the resultant potential for creating oscillations.
The exact mechanisms operating in any particular cell type, to generate any particular oscillatory pattern, are extraordinarily difficult to determine. In most cases the oscillatory mechanism is not certain.
Mathematical Mechanisms
Basic model structure
Models of calcium oscillations describe the various calcium fluxes from and into the cytoplasm. Given the simple structure shown at right, conservation of calcium gives the equations \[ {dc\over dt} = J_{\rm release} - J_{\rm serca} + J_{\rm influx} - J_{\rm pm} \] \[ {dc_e\over dt} = \gamma(J_{\rm serca} - J_{\rm release}), \] where \(c\) denotes the concentration of calcium in the cytoplasm, and \(c_e\) denotes the concentration of calcium in the ER (or SR). Additional fluxes such as mitochondrial and buffer fluxes can be added in the same manner.
In general, the conservation equations for \(c\) and \(c_e\) are coupled to other equations that describe, for example, the states of the IPR, the states of the ATPase pumps, the calcium buffers, and the concentration of IP_{3} (Goldbeter et al., 1999; Atri et al., 1993; De Young and Keizer, 1992; Dupont et al., 1991, 2003; Falcke, 2003; Hofer, 1999).
The parameter \(\gamma\) denotes the ratio of the cytoplasmic volume to the ER volume. This is necessary as the concentrations \(c\) and \(c_e\) refer to different volumes. Since the volume of the ER is less than that of the cytoplasm, the flow of calcium from the cytoplasm to the ER will cause a greater concentration change in the ER than it does in the cytoplasm.
This style of model assumes that the concentration of calcium is the same throughout the cell, i.e., that the cell is well-mixed.
Modelling the fluxes
To construct a specific model of calcium oscillations it remains only to choose particular models of each of the fluxes and substitute them into the evolution equations. Practically any reasonable choice of model fluxes will lead to oscillations, of one type or another, for at least a range of parameter values.
Construction of models of each of these fluxes is an art form all to itself; models of the IPR range from simple two-variable models to complex models with thousands of variables (Li and Rinzel, 1994; Sneyd and Falcke, 2004), SERCA models range from a simple Hill function to multistate Markov models (Keener and Sneyd, 1998), while no good mechanistic model has yet been constructed of the calcium influx from outside the cell (as we just do not know enough about it, as yet).
Open-cell and closed-cell models
In some cases it is reasonably accurate to assume that there is little flux of calcium across the membrane of the cell, and thus the total amount of calcium in the cell remains constant. In this case \[ \gamma c + c_e = {\rm constant}, \] and the model solutions live approximately on this line. Such models are called closed-cell models.
In cases where calcium fluxes across the plasma membrane are allowed no such constraint applies; such models are called open-cell models.
Often, the total amount of calcium in the cell can be treated as a slow parameter, as it is often the case that calcium transport to and from the outside of the cell is much slower than calcium transport to and from the internal compartments (Sneyd et al., 2004).
Stochastic models
In many cell types calcium oscillations do not occur with a regular period, but are clearly strongly influenced by stochastic processes (Marchant and Parker, 2001; Falcke, 2003). The exact nature of these stochastic processes is not always clear, but the most plausible explanation is that stochastic opening and closing of the IPR is the most important stochastic process causing irregularity in calcium oscillations. In fact, it can be shown that oscillations can occur in a stochastic model even when the underlying deterministic system is in a non-oscillatory state. This raises the possibility that many of the observed oscillations are actually a result of an underlying stochastic process rather than an underlying deterministic oscillatory process. Not surprisingly, it is difficult to distinguish between these hypotheses, and from an experimental point of view, the exact role of stochasticity remains to be determined.
Calcium Waves
In some cell types, calcium oscillations occur practically uniformly across the cell. In such a situation, measurement of the calcium concentration at any point of the cell gives the same time course, and a well-mixed model is appropriate. More often, however, each oscillation takes the form of a wave moving across the cell; these intracellular "oscillations" are actually periodic intracellular waves. To model and understand such spatially distributed behavior, inclusion of calcium diffusion is vital.
It is widely believed intracellular calcium waves are driven by the diffusion of calcium between calcium release sites. According to this hypothesis, the calcium released from one group of release sites (usually either IPR or RyR) diffuses to neighboring release sites and initiates further calcium release from them. Repetition of this process can generate an advancing wave front of high calcium concentration, i.e., a calcium wave. Since they rely on the active release of calcium via a positive feedback mechanism, such waves are actively propagated.
However, when the underlying calcium kinetics are oscillatory (i.e., there is a stable limit cycle), waves can propagate by a kinematic, or phase wave, mechanism, in which the wave results from the spatially ordered firing of local oscillators. These waves do not depend on calcium diffusion for their existence, but merely on the fact that one end of the cell is oscillating with a different phase than the other end. In this case, calcium diffusion serves to synchronize the local oscillators, but the phase wave persists in the absence of calcium diffusive coupling.
It is usually (but not always) assumed that calcium diffuses with constant diffusion coefficient D_{c}, and that the cellular cytoplasm is isotropic and homogeneous. We also assume that \(c\) and \(c_e\) coexist at every point in space. Obviously this is not what really occurs. However, if the ER is "smeared out" sufficiently, it is reasonable to assume that every point in space is close enough to both the ER and the cytoplasm to allow for such a simplified model. This type of smeared model can be rigorously derived using homogenisation theory.
In this case, the reaction-diffusion equation for Ca^{2+} is \[ {\partial c\over \partial t} = D_c\nabla^2 c + J_{\rm release} - J_{\rm serca}. \] Note that the fluxes \(J_{\rm in}\) and \(J_{\rm pm}\) are omitted as they are fluxes only on the boundary of the cellular domain and must be treated differently. \(D_c\) is an effective diffusion coefficient that can be derived using homogenisation.
ER calcium
It is not known how well Ca^{2+} diffuses in the ER, or the extent to which the tortuosity of the ER plays a role in determining an effective diffusion coefficient for ER Ca^{2+}. It is typical to assume either that Ca^{2+} does not diffuse in the ER, or that it does so with a restricted diffusion coefficient, \(D_e\ .\) In either case, \[ {\partial c_e\over \partial t} = D_e\nabla^2 c_e - \gamma (J_{\rm release} - J_{\rm serca}), \] with \(0<D_e\le D_c\ .\)
Boundary conditions
Cells are inherently three dimensional objects. When cells are viewed as three dimensional the boundary conditions are clear. At the boundary of the cell (i.e., the plasma membrane) the flux per unit area into the cell is \(J_{\rm in} - J_{\rm pm}\ .\) Thus, \[ \begin{matrix} D_c \nabla c \cdot n &=& J_{\rm in} - J_{\rm pm}, \\ \nabla c_e \cdot n &=& 0, \end{matrix} \] where \(n\) is the outward unit normal to the boundary.
However, if the cell is viewed as a long one dimensional object, with calcium homogeneously distributed in each cross-section, then the fluxes across the (cylindrical) wall must be included as source terms in the governing partial differential equation. For example, for a "one-dimensional" cell of length \(L\) with no calcium flux at the ends, the boundary conditions are \({\partial c \over \partial x} = 0\) at \(x=L\) and \(x=0\ ,\) while at each internal point we have \[ {\partial c\over \partial t} = D_c{\partial^2 c\over \partial x^2} + {\rho\over A}(J_{\rm in} - J_{\rm pm}) + J_{\rm release} - J_{\rm serca}. \] where \(\rho\) is the cell perimeter and \(A\) the cell cross-sectional area.
There are a number of ways to study reaction-diffusion models of this type, but the two most common are numerical simulation or bifurcation analysis of the traveling wave equations.
Bifurcation analysis
If we introduce the traveling wave variable \(\xi=x+st\ ,\) where \(s\) is the wave speed we can write the equation for calcium diffusion as the pair of equations \[ \begin{matrix} c' &=& d, \\ D_cd' &=& sd - \sum J, \end{matrix} \] where \(\sum J\) denotes all the calcium fluxes, and where a prime denotes differentiation with respect to \(\xi\ .\) These two equations are coupled to the other equations for \(c_e\ ,\) \(p\ ,\) and the states of the various receptors. When all the unknown variables are written as functions of the variable \(\xi\ ,\) these equations are called traveling wave equations.
Traveling pulses, traveling fronts, and periodic waves correspond to, respectively, homoclinic orbits, heteroclinic orbits and limit cycles of the traveling wave equations. However, such an approach does not readily give information about the stability of the wave solutions of the original reaction-diffusion equations.
References
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- M. J. Berridge, (1990). Calcium oscillations. Journal of Biological Chemistry, 265, pp. 9583--9586
- M. J. Berridge, (1997). Elementary and global aspects of calcium signalling. J Physiol, 499 ( Pt 2), pp. 291-306
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- S. Schuster, M. Marhl and T. Hofer, (2002). Modelling of simple and complex calcium oscillations. From single-cell responses to intercellular signalling. Eur J Biochem, 269, pp. 1333-55
- J. Sneyd and M. Falcke, (2004). Models of the inositol trisphosphate receptor. Prog Biophys Mol Biol, pp. in press
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- A. P. Thomas, G. S. J. Bird, G. Hajnóczky, L. D. Robb-Gaspers and J. W. J. Putney, (1996). Spatial and temporal aspects of cellular calcium signaling. FASEB J, 10, pp. 1505--1517
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Internal references
- John Guckenheimer (2007) Bifurcation. Scholarpedia, 2(6):1517.
- James Meiss (2007) Dynamical systems. Scholarpedia, 2(2):1629.
- Jeff Moehlis, Kresimir Josic, Eric T. Shea-Brown (2006) Periodic orbit. Scholarpedia, 1(7):1358.
- Gregoire Nicolis and Anne De Wit (2007) Reaction-diffusion systems. Scholarpedia, 2(9):1475.
- Philip Holmes and Eric T. Shea-Brown (2006) Stability. Scholarpedia, 1(10):1838.
- Arkady Pikovsky and Michael Rosenblum (2007) Synchronization. Scholarpedia, 2(12):1459.
External links
See also
Bifurcations, Bistability, Dynamical Systems, Excitable Media, Reaction-Diffusion Systems, Stability, Traveling Wave